Derivative Assay:Article Title: MITF, TFEB, and TFE3 drive distinct adaptive gene expression programs and immune infiltration in melanoma.
Article Snippet: .. Obtained from Ruth Halaban, Yale Zakut et al. 109 A375M Human melanoma cell line (male) Obtained from ATCC Giard et al. 110 SKMEL28 Human melanoma cell line (male) Obtained from ATCC Fogh et al. 111 SKMEL30 Human melanoma cell line (female) Obtained from ATCC Carey et al. 112 HBL Human melanoma cell line (female) Obtained from Ghanem Ghanem, Brussels Ghanem et al. 113 WM793 Human melanoma cell line (male) Obtained from the ATCC Herlyn et al. 114 WM266.4 Human melanoma cell line (female) Obtained from the ATCC Westermark et al. 115 WM115 Human melanoma cell line (female) Obtained from the ATCC Westermark et al. 115 WM9 Human melanoma cell line (male) Obtained from the ATCC Herlyn et al. 116 MeWo Human melanoma cell line (male) Obtained from the ATCC Cornil et al. 117 CHL-1 Human melanoma cell line (female) Obtained from the ATCC (Derived from RPMI 7932 cells) Patent US5017478A HT-29 Human Colon Cancer (female) Obtained from the ATCC Fogh and Trempe 118 B16-F10-Luciferase mouse melanoma cell line (male) Obtained from Luis Sanchez del Campo Sanchez-Del-Campo et al. 81 B16-F10-Luciferase MITF KO mouse melanoma cell line (male) Obtained from Luis Sanchez del Campo Sanchez-Del-Campo et al. 81 Experimental Models: Mice Female C57BL/6 Envigo (Barcelona, Spain) N/A (Continued on next page) Cell Reports 44, 116499, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides siRNA control Qiagen Cat# 1027281 siRNA MITF 1 5 ′ -AAAGCAGTACCTTTCTACCAC Sigma Falletta et al. 25 siTFE3 Santa Cruz Cat# sc-38507 TFEB sgRNA 5 ′ -AGTACCTGTCCGAGACCTAT ThermoFisher A35533 CRISPR775049_SGM This paper TFE3 sgRNA 5 ′ -GGTACTGTTTCACCTGCTGC ThermoFisher A35533 CRISPR381077_SGM This paper Tfeb sgRNA 5 ′ -TGGACACGTACTGTCCACCT ThermoFisher A35533 CRISPR557759_SGM This paper Tfe3 sgRNA 5 ′ -AGCGTGTAGGGTTCTCGAGG ThermoFisher A35533 CRISPR284202_SGM This paper DEPTOR qRT PCR Forward 5 ′ -ACTCATGACAGTCCCTTCTGC N/A This paper DEPTOR qRT PCR Reverse 5 ′ -GACACGATCTTGATCTCCTTGCT N/A This paper PTEN qRT PCR Forward 5 ′ -TGGCACTGTTGTTTCACAAGAT N/A This paper PTEN qRT PCR Reverse 5 ′ -TAGCTGGCAGACCACAAACT N/A This paper MITF qRT PCR Forward 5 ′ -AGGTACTTTGATTCCCAAGTCA N/A This paper MITF qRT PCR Reverse 5 ′ -AAGTTCTTTTGCGCGTTGCT N/A This paper Recombinant DNA Plasmid Piggybac expression system N/A Magnú sdó ttir et al. 119 Software and algorithms MetaQuant N/A Bunk et al. 120 ImageJ https://imagej.nih.gov/ij/ N/A Cutadapt N/A Martin 121 STAR N/A Dobin et al. 122 Homer N/A Heinz et al. 123 EdgeR N/A Robinson et al. 124 TreeView N/A Saldanha et al. 125 javaGSEA v.4.3.2 N/A Subramanian et al. 126 emmeans v1.10.7 cran.r-project.org N/A nlme v.3.1–166 cran.r-project.org N/A fastqc v.0.11.9 GitHub - s-andrews/FastQC N/A trim_galore v.0.6.5 N/A Martin et al. 121 clusterProfiler v4.6.2 Bioconductor N/A DOSE v3.24.2 Bioconductor N/A enrichplot v.1.18.4 Bioconductor N/A VennDiagram v.1.7.3 cran.r-project.org N/A ggplot2 v.3.4.4 cran.r-project.org N/A GraphPad Prism 7 https://www.graphpad.com N/A Perseus N/A Tyanova et al. 127 22 Cell Reports 44, 116499, December 23, 2025
Mouse Assay:Article Title: MITF, TFEB, and TFE3 drive distinct adaptive gene expression programs and immune infiltration in melanoma.
Article Snippet: .. Obtained from Ruth Halaban, Yale Zakut et al. 109 A375M Human melanoma cell line (male) Obtained from ATCC Giard et al. 110 SKMEL28 Human melanoma cell line (male) Obtained from ATCC Fogh et al. 111 SKMEL30 Human melanoma cell line (female) Obtained from ATCC Carey et al. 112 HBL Human melanoma cell line (female) Obtained from Ghanem Ghanem, Brussels Ghanem et al. 113 WM793 Human melanoma cell line (male) Obtained from the ATCC Herlyn et al. 114 WM266.4 Human melanoma cell line (female) Obtained from the ATCC Westermark et al. 115 WM115 Human melanoma cell line (female) Obtained from the ATCC Westermark et al. 115 WM9 Human melanoma cell line (male) Obtained from the ATCC Herlyn et al. 116 MeWo Human melanoma cell line (male) Obtained from the ATCC Cornil et al. 117 CHL-1 Human melanoma cell line (female) Obtained from the ATCC (Derived from RPMI 7932 cells) Patent US5017478A HT-29 Human Colon Cancer (female) Obtained from the ATCC Fogh and Trempe 118 B16-F10-Luciferase mouse melanoma cell line (male) Obtained from Luis Sanchez del Campo Sanchez-Del-Campo et al. 81 B16-F10-Luciferase MITF KO mouse melanoma cell line (male) Obtained from Luis Sanchez del Campo Sanchez-Del-Campo et al. 81 Experimental Models: Mice Female C57BL/6 Envigo (Barcelona, Spain) N/A (Continued on next page) Cell Reports 44, 116499, December 23, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides siRNA control Qiagen Cat# 1027281 siRNA MITF 1 5 ′ -AAAGCAGTACCTTTCTACCAC Sigma Falletta et al. 25 siTFE3 Santa Cruz Cat# sc-38507 TFEB sgRNA 5 ′ -AGTACCTGTCCGAGACCTAT ThermoFisher A35533 CRISPR775049_SGM This paper TFE3 sgRNA 5 ′ -GGTACTGTTTCACCTGCTGC ThermoFisher A35533 CRISPR381077_SGM This paper Tfeb sgRNA 5 ′ -TGGACACGTACTGTCCACCT ThermoFisher A35533 CRISPR557759_SGM This paper Tfe3 sgRNA 5 ′ -AGCGTGTAGGGTTCTCGAGG ThermoFisher A35533 CRISPR284202_SGM This paper DEPTOR qRT PCR Forward 5 ′ -ACTCATGACAGTCCCTTCTGC N/A This paper DEPTOR qRT PCR Reverse 5 ′ -GACACGATCTTGATCTCCTTGCT N/A This paper PTEN qRT PCR Forward 5 ′ -TGGCACTGTTGTTTCACAAGAT N/A This paper PTEN qRT PCR Reverse 5 ′ -TAGCTGGCAGACCACAAACT N/A This paper MITF qRT PCR Forward 5 ′ -AGGTACTTTGATTCCCAAGTCA N/A This paper MITF qRT PCR Reverse 5 ′ -AAGTTCTTTTGCGCGTTGCT N/A This paper Recombinant DNA Plasmid Piggybac expression system N/A Magnú sdó ttir et al. 119 Software and algorithms MetaQuant N/A Bunk et al. 120 ImageJ https://imagej.nih.gov/ij/ N/A Cutadapt N/A Martin 121 STAR N/A Dobin et al. 122 Homer N/A Heinz et al. 123 EdgeR N/A Robinson et al. 124 TreeView N/A Saldanha et al. 125 javaGSEA v.4.3.2 N/A Subramanian et al. 126 emmeans v1.10.7 cran.r-project.org N/A nlme v.3.1–166 cran.r-project.org N/A fastqc v.0.11.9 GitHub - s-andrews/FastQC N/A trim_galore v.0.6.5 N/A Martin et al. 121 clusterProfiler v4.6.2 Bioconductor N/A DOSE v3.24.2 Bioconductor N/A enrichplot v.1.18.4 Bioconductor N/A VennDiagram v.1.7.3 cran.r-project.org N/A ggplot2 v.3.4.4 cran.r-project.org N/A GraphPad Prism 7 https://www.graphpad.com N/A Perseus N/A Tyanova et al. 127 22 Cell Reports 44, 116499, December 23, 2025
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